College of Medicine Research Core Facilities
The UC College of Medicine houses a number of research core facilities designated as core service centers. These facilities exist within multiple departments but are collectively supported by the College of Medicine Office of Research through the Associate Dean for Research Core Facilities: Ken Greis, PhD. (ken.greis@uc.edu; Tel: 513-558-7012).
The service center designation signifies the rates charged by each of these facilities have been reviewed and approved by the UC Government Cost Compliance Office; thus, the service fees can be cross-charged to federal grants and contracts. Details related to the services offered and the internal rates for each of the cores are provided below. Since these rates are substantially subsidized by the University, external investigators should contact individual core directors to get a rate quote.
Resources to offset some of the cost of the core services may be available through a variety of centers and institutes across UC depending on an investigator’s affiliation. Information related to some of those opportunities and the website links are provided below:
- Center for Clinical and Translational Science and Training (CCTST) Just-in-Time Core Support
- Center for Environmental Genetics (CEG) Integrative Technologies Support
We have recently transitioned our core facilities booking and management to the PPMS system from Stratocore. To book and access services from the core facilities, please log in or create an account in Stratocore via:
Stratocore Account Creation Guides:
UC Flow Cytometry Core
The Flow Core supports a Beckman CytoFLEX S with 9 detectors and 4 lasers in CARE 2874 and a FACSCalibur 4 color flow cytometer system in CARE 2876. Access is available 24 hrs a day and 7 days per week once investigators are registered in Stratocore and approved by the core director.
To book equipment and/or access services from the Flow Core, please login or create an account in Stratocore at https://ppms.us/uc/start/.
For access to the instruments and the online calendar, please contact Dr. Miller.
Grant Information
A general NIH description of facilities and equipment for this core may be accessed with this link - UC Flow Cytometry NIH Summary May 2025; however, it is highly recommended that you discuss your specific core needs with the core director or manager while preparing the grant application since they can likely provide tailored information regarding their capabilities to enhance your application.
| Service | Cost |
|---|---|
|
CytoFLEX S System Access (4 laser, 9 color) |
$20 per half hour |
|
FACSCalibur System access (2 Laser, 4 color) |
$20 per half hour |
FACSCalibur Four Color Flow Cytometer
The FACSCalibur is configured as follows:
- FL1 Channel - Blue Laser (488nm) and 515-545nm Emission Filter (FITC, GFP, etc.)
- FL2 Channel - Blue Laser (488nm) and 564-601nm Emission Filter (PE, PI, RFP, etc.)
- FL3 Channel - Blue Laser (488nm) and 670LP Emission Filter (7-AAD, PerCP, etc)
- FL4 Channel - Red Laser (635nm) and 653-669nm Emission Filter (APC, Cy5, etc.)
Beckman CytoFLEX S V2-B2-Y3-R2 Flow Cytometer (9 Detectors, 4 Lasers)
Here are the channels currently configured (in bold) the ones in italics can be utilized by repositioning the filters, which is relatively easy to do.
CytoFLEX (4 laser, 9 color)
Violet Laser (405nm) Emmision Filter
PB450 (PacBlue, eFluor450) 450/45
KO525 (Krome Orange, Zombie Aqua) 525/40
BV605 610/20
BV650 660/20
BV786 780/60
Blue Laser (488nm)
FITC (GFP, Alexa488, Venus) 525/40
PerCP (PC5, PC5.5) 690/50
Yellow/Green Laser (561nm)
PE (PI, Sytox Orange, DsRed, TdTomato) 585/42
ECD (mCherry, Texas Red) 610/20
PC7 780/60
PC5 690/50
Red Laser (638nm)
APC (AlexaFluor 647, eFluor660) 660/10
APC-A750 (APC-Cy7, APC eFluor780 780/60
APC-A700 (AlexaFluor 700)
Location
CARE 2874-2876
213 Albert Sabin Way
Cincinnati
45267-0524
Contact Information
- William Miller PhD
- william.miller@uc.edu
- 513-558-0866
Contact Us
University of CincinnatiCollege of Medicine
CARE/Crawley Building
Suite E-870
3230 Eden Avenue
PO Box 670555
Cincinnati, OH 45267-0555
Mail Location: 0555
Phone: 513-558-7333
Fax: 513-558-3512
Email: College of Medicine